fit gel into the inner frame. The long gel plate is FACING TOWARDS YOU, and the shorter one is facing away from you.
It is important to get a tight seal between the green gasket and the white frame of the gel. The gel and the buffer dam on the other side will form the inner buffer chanber.
|The buffer dam and the gel form the inner buffer
chamber. The frame is then slid into the support. Note that the gel is
held in place to get a tight seal. Once the frame is inserted, the levers
are closed to LOCK the frame in place. The levers must be closed at
the same time. You should get some resistance.
|Now the assembled support and frame are lowered into the outer buffer chanber. Remember that the gel and the buffer dam on the other side form the inner buffer chanber.|
|the assembled acrylamide gel rig. Next, buffer will be added to the inner chamber to check for a tight seal.|
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